Tailor-Made Variants of Site-Specific Recombinases as Tools for Genome Engineering
The objective of the investigator of this research is to develop an efficient and universal genome manipulation technology through tailor-made variants of the site-specific recombinase Flp. This may be accomplished through: identifying and classifying all human genome sequences resembling the Flp target, known as FRT, evolving Flp variants capable of recombining with FRT-like sequences, and developing a selection method to be used in identifying successful site-specific recombination. This research may also include the correction of disease-causing mutations in model cell lines representing sickle cell disease, cystic fibrosis, Tay-Sachs syndrome, and Lesch-Nyhan syndrome by targeting FRT-like sites within the mutations, ultimately replacing the mutations in the cell lines.
Principal Investigator: Voziyanov, Yuri -- Biological Sciences
|Amount Awarded: 1,238,896
|Start Period: 08/01/2008
||End Period: 05/31/2014