Long-Term 3D Imaging of Mouse Brain In-Vivo to Study Glial Cells and Gliogenesis
In an effort to advance research efforts in drug addiction, stem cell therapy, neurodegeneration, and other areas, the investigator purposes to develop a novel implantable lens capable of studying labeled glial cells in mice. Based on a design developed by the investigator, the proposed research plan is to manufacture a corrected, thin, gradient index lens (ctGRIN) with corrective windows and positive spherical aberration capable of producing high resolution images. By creating a single lens system, fewer mice may be required for research, mice health will be greatly improved, and higher resolution images can be produced. After labeling glial cells in the hippocampus of mice subjects with Green Fluorescence Protein, the cells will be subjected to a calcium-sensitive dye and astrocyte-specific dye. The ctGRIN lens will then be used to create 3D in vivo images that will be used to observe labeled cell interactions. Data collected from this project may lead to significant contributions in medical research and therapeutic efforts in individuals affected by neurodegenerative diseases, drug addictions, and other areas associated with neural functions.
Principal Investigator: Murray, Teresa -- Biomedical Engineering/CBERS
|Start Period: 09/10/2012
||End Period: 08/31/2015