The Role of Mediator in Regulating the State of Mesenchymal Stem Cells
A major area of contemporary research devoted to the treatment of human disease is the manipulation of pluripotent and multipotent stem cells capable of differentiating into many tissue types. This project will examine a multi-subunit protein complex known as Mediator, which functions to retain the pluripotency of mesenchymal stem cells (MSCs) through the management of gene expression in mice. In this research, the investigators will learn techniques required to appropriately culture human MSCs. Through the establishment of current methods of culturing these stem cells, the investigators will then be allowed to study other characteristics of the Mediator complex such as: subunit expression, establish western blot assays for human MSC transcription factors, and the effects of Mediator and transcription factor losses by knockdown. These steps will allow for the identification of genes dependent on Mediator for expression. Data collected from this project has the potential to provide greater insight in stem cell research and human disease treatment through stem cell manipulation.
Principal Investigator: Newman, Jamie -- Biological Sciences
|Start Period: 01/01/2014
||End Period: 12/31/2014